Avaliação dos parâmetros inflamatórios de baixo grau, bioquímicos e de estresse oxidativo em ratos com dieta rica em frutose

Abstract

The study aimed to determine the effects of fructose intake on low-grade inflammatory, biochemical, and oxidative stress parameters in rats subjected to a high-fructose diet. A total of 40 animals, aged 30 days, were divided into four groups: the control group (C), which received standard chow and water, and the treated group, which was subdivided into three groups: (1) standard chow with 40% fructose supplementation, (2) standard chow with 30% fructose supplementation, and (3) standard chow with 20% fructose supplementation, for sixty days. There were no food or water restrictions. Mieloperoxidase (MPO), high-sensitivity C-reactive protein (hs-CRP), and blood glucose levels did not show statistically significant changes. However, there were statistically significant alterations in creatinine levels (p < 0.001) in the 40% and 30% groups and (p < 0.0001) in the 20% group, with the same significance for the glomerular filtration rate (GFR). Uric acid levels showed significant differences in the 40% (p < 0.001) and 30% (p < 0.05) groups. Total cholesterol significantly increased (p < 0.001) in the 40% group and (p < 0.0001) in the 30% and 20% groups. Triglyceride levels increased (p < 0.001) in the 40% group and (p < 0.01) in the 30% and 20% groups. Aspartate aminotransferase (AST) showed statistical significance (p < 0.05) in the 20% group, while alanine aminotransferase (ALT) increased (p < 0.01) in the 30% group and (p < 0.001) in the 20% group compared to the control group. Additionally, the rates of substances reactive to thiobarbituric acid (TBA-RS) in liver tissue showed statistical significance (p < 0.05) in the 40% group and (p < 0.01) in the 20% group. In kidney tissue, significance was observed in the 40% group (p < 0.05). Therefore, the study protocol demonstrated metabolic alterations in hepatic and renal functions, as well as changes related to oxidative stress.